Tuesday, May 31, 2011

Conferences, meme-sex and some science for good measure...

This post was co-authored by Kiyoko and Ben (equal contributors). Photos are merely eye-candy.

This blog is generally about eco-evolutionary dynamics, especially involving contemporary evolution (rapid microevolution, if you will). Recently, the Sixth Annual Congress of the Canadian Society for Ecology and Evolution (CSEE) was held in Banff, Alberta. Given the theme of this blog, we thought we’d present some musings on the eco-evolutionary dynamics of grad students and conferences.

            A key component of eco-evolutionary dynamics is genetics, genes being the primary way that organisms transmit biological information to future generations. Grad students, however, aren’t passing on genes (well, maybe they are, but not in public), but memes. We’re transmitting ideas and beliefs to each other. During talks, at lunches and dinners, and even during coffee breaks, the primary activity that defines a conference is the passing of information. This information can range from tips for surviving grad school, to a new statistical method for data analysis, to gossip about who just got rejected from Science (and, more juicy still, why). Students are sometimes still looking for ideas for a Masters or PhD, profs are looking for new lab members, and governmental organizations are on the prowl as well. CSEE was particularly conducive to the lateral transfer of memes, with fabulously catered coffee breaks spread out on different floors, as well as breakfasts and lunches for all attendees in one common area. The local pubs of Banff also proved an excellent place for ‘meme-sex’; everybody knows that the exchange of heritable information goes more smoothly after a beer or two.  Microbrews speed microevolution!

            Camouflage and mimicry play a major part in these eco-evolutionary dynamics, as we consciously seek to blend into our academic surroundings so that we don't get picked off by predators (with those pesky un-answerable questions!).  At the same time, though, we want to have sparkly new ideas that others like, and that attract collaborators to us.  Thus, we are torn between natural selection for crypsis and sexual selection for conspicuousness, tossed about wildly by the unpredictable vagaries of a spatiotemporal mosaic of selection. Such opposing selective forces are perhaps best negotiated via phenotypic plasticity, although many graduate students find other bet-hedging strategies. A lot of our traits are inherited from our advisors, with some variation, giving the process a distinctly Darwinian demeanour.  However, our advisors choose us and we choose our advisors so it is perhaps also a little Lamarckian: traits that our advisors have found useful during their careers tend to be encouraged in their offspring. For instance, Andrew likes to stick his neck out to reach tasty leaves. Perhaps he will turn into a giraffe by the time we finish our theses.

            Conferences like CSEE are where graduate students go to have meme-sex. And CSEE was HOT! We promiscuously mixed our ideas together, allowing them to recombine freely, and we dreamt up new, wild and wonderful concepts that we will now pursue alone in our basement offices.  Some graduate students have actual sex at conferences, and some of those end up married as a result; but probably many more have "meme-sex" and end up in lifelong collaborations as a result. Please, don’t confuse your model organism with your offspring. Just remember the top three differences: (1) granting agencies are unlikely to fund the development of your children; (2) Drosophila do not wear diapers; and (3) naming your children and becoming emotionally attached to them – even anthropomorphizing them! – is generally encouraged.

            So what kind of meme-sex happened? As an example, let’s see what happened to Kiyoko’s project, which looks at spatiotemporal variation in selection using the Trindadian guppies (now you get the above references, right?). She got great feedback on her talk on spatiotemporal variation in colour patterns in guppies, including ideas on possible sources and drivers of this variation. Other lateral meme transfer came from posters and talks on topics ranging from opsin genes to visual perception based on cone sensitivities to potential physiological costs of producing colour pigments. She got to bounce ideas for future experiments off of other folks, and even sniffed out a few potential collaborations for the future.

So where does that leave us? Nowhere really, except to go back to our office dwellings and cultivate these new memes we’ve absorbed. We will evolve and grow and differentiate, and by the next conference, we’ll have a new batch of memes prepared, ready to be, uh, sprayed indiscriminately at our colleagues. Well, sometimes analogies can be taken too far; we apologize for that last mental image. Next year’s congress has been unofficially dubbed Evo-palooza, as it will be a joint conference including CSEE, SSE, ASN, SSB, and ESEB! That will be an incredibly stimulating conference. Don’t forget a condom!

P. S. We saw a bear!

Tuesday, May 17, 2011

Eco-evolutionary Trophic Dynamics and the Loss of Top Predators

Humanity is at odds with the world’s top predators. Ecologists have long recognized the importance of top predators for the functioning of food webs. Decades of work have revealed that top predator removal can impact the biomass of primary producers. This is because, as top predators decline, prey populations increase, initiating trophic cascades. Traditional trophic cascades are mediated by the demographic and behavioral responses of prey populations. But predator removal may also have important effects on prey evolution. When predators are present and prey density is low, natural selection may favor prey traits that are important for predator escape ability. However, when predators are eliminated and prey density increases, natural selection may shift, now favoring traits that are important for competitive ability. This shift in natural selection may modify important trophic interactions.

Mike Kinnison, Ben Wasserman and I investigated the impact of predator loss on the evolution and ecology of prey. We took advantage of a historical introduction experiment involving Trinidadian guppies. In 1976, John Endler introduced about 200 guppies from a site with the top fish predator Crenicichla to a site lacking predators. Much is known about how this introduced guppy population has evolved in terms of color patterns and life history traits. However, little is known about how trophic morphology and feeding rates have changed in response to predator loss. We hypothesized that the absence of Crenicichla would lead to increased guppy density and heightened intraspecific competition. Due to trade-offs between gathering resources and avoiding predators, we predicted that the population released from predation would display heightened feeding rates compared to the high-predation source population.

Our results confirmed this prediction. The introduced population and a nearby natural low-predation population both displayed greater guppy densities and higher individual level consumption rates than the high predation source population. In addition, morphometric analysis revealed that both head and body shape have evolved to facilitate heightened resource acquisition. Results from prior experiments in mesocosms suggest that heightened feeding rates in low-predation guppy populations may cause them to have stronger top-down effects on algal biomass compared to high-predation populations.

Traditionally, the loss of top predators has been considered from a strictly ecological point of view. Our results suggest that predator loss may drive prey evolution, which itself may have important ecological effects – in this case, amplifying the strength of trophic cascades. If our results reflect a common response of prey populations to the loss of top predators, then a full assessment of the ecological impacts of top predator removal must carefully consider the effects of prey evolution.

This study recently appeared in PLoS ONE: http://dx.plos.org/10.1371/journal.pone.0018879.

Thursday, May 5, 2011

Suskewiet! Finchy parallelisms between intended selection by domestication and unintended alterations of the strength of natural selection.

The dutch word in this title probably needs translation. “Suskewiet“ is an onomatopoeia (=a sound-imitating word) imitating the last part of the song of the male chaffinch (Fringilla coelebs), a small passerine bird in the finch family Fringillidae. Sorry, this is not entirely correct. “Suskewiet” only refers to the last part of the song as it is performed in Flanders, Belgium. Indeed, the chaffinch occurs all over Europe, parts of Asia, and North Africa, and there is considerable geographical variation in song. So “suskewiet” belongs to the repertoire of the birds’ Flemish dialect.

The male of the chaffinch.

Why would one need a specific word to talk about the last part of the song of Flemish chaffinches? This is a long story – but the picture below tells it all:

A “vinkenzetting”, or finch championship, in Flanders (Belgium).

Silence please! Here you see an important folkloristic competition going on, called “vinkenzetting” (finch championship) . Each of the boxes contains a male finch, and the championship is all about which finch makes this precise “suskewiet” sound most often. The men and women in the picture are holding a pole and a piece of chalk, to keep track of the number of suskewiet’s. The birds can hear each other, and their territorial nature makes them sing “suskewiet” as much as possible. The best finches sing more than 600 times per hour! The owner of the best finch wins a symbolic price, and the finch becomes more valuable. The tradition goes back to the Middle Ages (first mentioning of a finch game in 1595), and, despite or thanks to its weirdness, still persists. People practicising the game are called “vinkeniers” (“finchers”). They used to have a pretty bad reputation for illegal practises such as blinding the birds (up to the 1900’s it was thought that blind birds sing more) and depleting the wild finch population with rude catching techniques (up to the 1970’s). Luckily, the finchers are now organised in an official federation (http://www.avibo.be/home.php), controlling the games with strict regulations, using domesticated birds only.

Just like farmers who know how to breed cattle, finchers have an impressive knowledge about how to breed finches (including the inheritance of interesting traits such as song and colour). I’m not sure how much of the variation in song is heritable, but breeders do select fathers based on song quality. However, it is possible to teach a male bird the right song exposing them to “teacher birds” or audiorecords. Interestingly, a bird not singing the Flemish “suskewiet” is called a “francophone”, making a supposedly less elegant “suskeweiih” noise. Talking about Belgium (and Quebec), this sounds a bit politically incorrect. However, birds have a right wing and a left wing, so they are probably politically neutral.

Returning from the Galapagos field expedition 2011, it came to my mind that male Darwin’s finches use neither “suskewiet” nor “suskeweiih” in their songs. This is no surprise, as Darwin’s finches and the chaffinch are not related. Their songs are actually very different, even though they have similar beaks.

Darwin probably would not be surprised to read that people are weird enough to domesticate finches (such as chaffinch and zebra finch), applying his theory of selection by domestication. He also wouldn’t be surprised to read that Darwin’s finches have become iconic for his theory on natural selection. But he might be surprised to read that humans can alter the strength of natural selection in Darwin’s finches. During previous expeditions, the Hendry lab has been investigating this possibility in the seed-eating medium ground finch (Geospiza fortis) from Santa Cruz island, by comparing the morphology of a population living at Academy Bay, a human-impacted site, with a population living at El Garrapatero, a natural site. G. fortis birds from Academy Bay had smaller beak size than birds from El Garrapatero. This probably implies that the presence of humans has caused a shift in the finches’ resource distribution by the introduction of human food (such as bread, rice and potato chips) or new plant species into the environment, creating a selective advantage for finches with smaller beaks.

Chaffinches in captivity and Darwin’s finches in the wild thus seem to have in common that humans can influence which finches are going to contribute to the next generation. In the first case we talk about intended selection by domestication, in the second case about unintended alteration of the strength of natural selection. Is it exaggerated to describe the latter as “unintended domestication”?

So far, indications that humans might influence the evolution of Darwin’s finches have only been observed in a single species. However, as scientists we don’t want to rely on a single significant P-value - just as a fincher is not satisfied with a single “suskewiet”. During the 2010 and 2011 expeditions, we measured five additional species at the human-impacted and the natural site study site: the small ground finch (Geospiza fuliginosa), the large ground finch (Geospiza magnirostris), the cactus finch (Geospiza scandens), the vegetarian finch (Platyspiza crassirostris), and the small treefinch (Camarhynchus parvulus). So, let’s have a look at the potential human impact on the beak morphology of these species. P-values are not very suitable here - as finchers wouldn’t understand them. Luckily , “suskewiet” sounds like a significant result (P < 0.05), whereas “suskeweiih” sounds perfect for an non-significant result (P > 0.05) – and finchers do understand it. This opens opportunities for a new “suskewiet vs. suskeweiih”-based school in statistics. Here I show how it works, testing for differences in beak length, beak depth and beak width, respectively, in of each of the investigated species.

The small ground finch – “Suskeweiih, suskeweiih, suskeweiih!.

Oops, bad start. We hear three times “suskeweiih”, as none of the three beak dimensions differs significantly between the population at the human-impacted site and the natural site. Actually, small ground finches seemed to have larger beaks at El Garrapatero in 2010, but this difference disappeared in 2011. But let’s go on, maybe we are more lucky with the next bird.

The vegetarian finch – “Suskeweiih, suskewiet, suskewiet!.

That’s a nicer song! Beak width and beak depth differ significantly between the sites, beak length does not. Beaks were larger at El Garrapatero, and this was consistent across years.

The large ground finch – “Suskeweiih, suskeweiih, suskeweiih!.

Awtsch, another bad song – this must be a francophone finch. No differences between the sites at all. I will have to sell this bird to another fincher.

The cactus finch – “Suskewiet, suskeweiih, suskeweiih!.

This song is a bit silent, but still elegant. There is a marginally significant difference for beak length, which was larger at El Garrapatero in both years. The other beak dimensions don’t differ.

The small treefinch – “Suskeweiih, suskewiet, suskewiet!.

Ha! Exactly the same song as the vegetarian finch. Beak width and beak depth differ significantly between the sites, beak length does not. Beaks were larger at El Garrapatero, and this was consistent across years.

So far so good. Now lets listen to the birds when they sing all together. It sounds harmonious because all suskewiet’s are generated by significantly larger beaks at El Garrapatero than at Academy Bay. So, the human-altered environment of Academy Bay seems to affect the bird species in the same way as observed previously for the medium ground finch. This makes sense, because the diets of these bird species partially overlap. A human-induced shift towards smaller and softer food items might thus select for smaller beaks in multiple bird species.

After the game finchers don’t go home early. They go to the local bar to talk about the championship and their finches, and to share their experiences or secrets. Science might benefit from this, so I might go there and ask them for advice. I'll probably start drinking to forget my disappointment in the ground finches, because both the large and the small one did not sing very well. In contrast, my cactus finch, small tree finch and vegetarian finch did a great job. This is changing my perspective. Small ground finches in particular are really keen on human food. Perhaps it does not affect the direction of selection in this species, because its natural diet might already be rather similar to the human food they can find. In contrast, I expected that cactus finches (feeding on cactus flowers), vegetarian finches (feeding on fruits) and small tree finches (mostly feeding on insects) would not bother about the introduction of novel food items by humans, and that their beak morphology would be conserved. However, my state-of-the-art “suskewiet vs. suskeweiih”-based statistical analyses suggested the opposite.

It is about time to bring back my finches to the aviary. It is worrying how rice, bread and potato chips might alter the live of a finch in the wild. In this sense there is sadness in all of the above songs. Let’s see how this is going to affect finch championships in the future.


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